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E. coli Plasmid Vectors

Methods and Applications
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Description

A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.
Release date NZ
November 10th, 2010
Audience
  • Professional & Vocational
Contributors
  • Edited by Andrew Preston
  • Edited by Nicola Casali
Edition
Softcover reprint of hardcover 1st ed. 2003
Illustrations
XII, 316 p.
Pages
316
Dimensions
155x235x18
ISBN-13
9781617373916
Product ID
9985819

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