Non-Fiction Books:

Visualizing RNA Dynamics in the Cell

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Description

Methods in Enzymology: Visualizing RNA Dynamics in the Cell continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods visualizing RNA dynamics in the cell, and includes sections on such topics as identification of RNA cis-regulatory sequences, IRAS, IMAGEtags, MERFISH, plant RNA labeling using MS2, and visualization of 5S dynamics in live cells using photostable corn probe.

Author Biography:

Dr. Grigory Filonov is a postdoctoral associate in the Department of Pharmacology at the Weill Medical College of Cornell University. He received a Ph.D. from the School of Chemistry of M.V. Lomonosov Moscow State University. Dr. Filonov conducted his first postdoctoral training at Albert Einstein College of Medicine of Yeshiva University where he engineered fluorescent proteins that can be used to visualize processes in live animals. Dr. Filonov then joined the laboratory of Dr. Jaffrey at Weill Cornell Medical College. Here Dr. Filonov is working on expanding the “RNA mimics of fluorescent proteins” technology. In particular, Dr. Filonov focuses on developing novel high-throughput screening approaches to isolate RNA-based fluorescent probes that have different spectral and photophysical properties. Additionally, Dr. Filonov is addressing the problem of robust expression of functional synthetic RNAs in live cells and organisms. Dr. Samie Jaffrey is a Professor of Pharmacology at the Weill Medical College of Cornell University. He received an M.D. and Ph.D. from Johns Hopkins School of Medicine where he also conducted postdoctoral work. Dr. Jaffrey’s laboratory focuses on identifying novel RNA regulatory pathways the control protein expression in normal cellular function and in disease processes. His research uses novel imaging, sequencing, microfluidic, and chemical biology approaches to address these questions. Dr. Jaffrey’s laboratory developed a novel class of RNAs referred to as “RNA mimics of green fluorescent protein,” which are used to image RNA localization and monitor RNA processing in living cells. The Jaffrey laboratory extended this technology to create a new class of genetically encoded biosensors composed of RNA that allows signaling molecules to be imaged in living cells.
Release date NZ
May 27th, 2016
Audience
  • Professional & Vocational
Contributors
  • Volume editor Grigory S. Filonov
  • Volume editor Samie Jaffrey
Pages
384
Dimensions
152x229x25
ISBN-13
9780128022924
Product ID
24541267

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