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Recombinant Protein Expression in Mammalian Cells on Hardback
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Recombinant Protein Expression in Mammalian Cells

Methods and Protocols
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Description

This detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either Chinese hamster ovary cells (CHO) or human embryonic kidney 293 cells (HEK293), the workhorses of the field, as the production host;  however, the methods can be adapted to other mammalian hosts under the appropriate cell-specific conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols aims to aid researchers in building on our knowledge of protein structure and function and to speed the discovery of new therapeutic proteins.
Release date NZ
September 22nd, 2018
Audience
  • Professional & Vocational
Contributor
  • Edited by David L. Hacker
Edition
1st ed. 2018
Illustrations
68 Illustrations, color; 8 Illustrations, black and white; XI, 311 p. 76 illus., 68 illus. in color.
Pages
311
ISBN-13
9781493987290
Product ID
28116704

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Books >
Non-Fiction >
Maths & Science >
Biology, life sciences >
Biochemistry >
Proteins
Books >
Non-Fiction >
Maths & Science >
Biology, life sciences >
Biochemistry