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Low-Cost Methods for Molecular Characterization of Mutant Plants

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Low-Cost Methods for Molecular Characterization of Mutant Plants

Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols
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Description

This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
Release date NZ
June 9th, 2015
Audience
  • Professional & Vocational
Illustrations
3 Illustrations, color; 6 Illustrations, black and white; X, 38 p. 9 illus., 3 illus. in color.
Pages
38
Dimensions
155x235x9
ISBN-13
9783319162584
Product ID
23144995

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