This book present the work that the study of extraction and analysis of heavy metals in fruit and vegetables by ICP MS. In the present study the concentrations of nine different heavy metals were determined in fruit and vegetable samples such as Grape, Pomegranate, Banana, Guava, Spinach and Onion which were freshly harvested in Pune region. Heavy metals were extracted from fruits and vegetables using Microwave digestion method and heavy metal concentrations were analyzed by using inductively coupled plasma mass spectrometry (ICP MS). The concentrations of As; Cd; Fe; Ni; Pb; Zn; Cr; Cu and Hg detected in samples. These values were lower than those recommended by FSSAI and WHO joint limits except Spinach (Cu 5.10) and Banana (Pb 0.26, As 1.5).