This handbook focuses on the operations which promise to produce genetically engineered materials for use in producing such end products as: enzymes, antibiotics, hormones, peptides, polypeptides, proteins, amino acids, veterinary drugs, steroids, herbicides, growth promoters, liposomes, diagnostic kits for infectious diseases, and many other materials that focus on pharmaceutical needs. The information in this volume should be of help to the chemical or pharmaceutical engineer who is developing a plant design and who faces such issues as: should the process be batch or continuous, or a combination of both?; how should the optimum process design be developed?; should one employ a new revolutionary separation which could be potentially difficult to validate, or use accepted technology which involves less risk?; should the process be run with ingredients formulated from water for injection, deionized water, or even filtered tap water?; should any of the separations be run in cold rooms or in glycol jacketed lines to minimize microbial growth where sterilization is not possible?