The development of plant structures like prickles involves a complex interplay of genetic pathways and regulatory networks. In this book, author used molecular analysis to identify potential gene networks and transcription factors regulating prickle development in Solanum viarum, a prickly plant species. The prickleless mutant was used as a model system to investigate the molecular mechanisms involved in prickle development. RNA sequencing and transcriptomic analysis were used to identify differentially expressed genes in the prickleless mutant compared to wild type plants. Further analysis of these genes revealed potential signaling pathways and genetic pathways involved in prickle development. Proteomic and metabolomic analysis were also used to identify potential protein-protein interactions and secondary metabolic pathways involved in the regulation of prickle development. To reconstruct gene networks involved in prickle development, gene co-expression and clustering analysis were performed, and gene ontology analysis was used to predict protein function. Pathway analysis was performed to identify potential pathways involved in prickle development, and the findings were validated by gene knockouts and other genetic manipulations. This book revealed the prickle development in Solanum viarum is regulated by a complex network of genes and pathways, including those involved in plant morphology, growth, and cell differentiation. Hormonal regulation also played a significant role, with plant hormones like auxin, cytokinin, abscisic acid, gibberellins, ethylene, and jasmonic acid being involved in the regulation of prickle development. Additionally, plant defense and secondary metabolism pathways were identified as playing a role in prickle development. The identification of potential gene networks and regulatory pathways involved in prickle development provides important insights into the molecular mechanisms underlying the development of plant structures; the information in this book could be used to develop new strategies for improving plant growth and development, as well as for crop breeding and genetic engineering.