The halophilic bacteria, isolated from Gulf of Khambhat, Gujarat, India. It's were characterized and diversified using the conventional approaches; colony characteristic, Gram reaction, cell morphology. The bacteria were Gram positive, rod shaped and catalase positive. Effect of incubation temperature, pH, NaCl were checked. Amylase, protease and lipase production was carried out. Majority of them produced lipase and protease, indicating their ecological a biotechnological significance. The bacteria were further categorized on the basis of the amplified ribosoma DNA restriction analysis (ARDRA) patterns, generated by a tetracutter RE, HaeIII. As a polyphasic taxonomy approach, the isolates were clustered into 9 different groups, based on the ARDRA patterns. As the studied habitat was not earlier explored for the bacterial diversity, the findings would significantly add to the knowledge of the cultivation based microbial diversity.