Non-Fiction Books:

Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis

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Description

This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an “in-situ” PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system’s performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successfuldevelopment and operation of this microsystem establishes the feasibility of rapid “sample-in-answer-out” testing in routine clinical practice.

Author Biography:

For internal review: Dr. Bin Zhuang Supervisor: Prof. Jing Cheng, School of Medicine, Tsinghua University Current Affiliation: National Engineering Research Center for Beijing Biochip Technology, Capitalbio Corporation Research Area: Biomedical engineering, microfluidic, nucleotide analysis, electrophoresis, biochemistry Publications: [1] Gan, W.P.,# Zhuang, B.,# Zhang, P.F., Han, J.P., Li, C.X., and Liu, P. (2014). A filter paper-based microdevice for low-cost, rapid, and automated DNA extraction and amplification from diverse sample types. Lab on a Chip 14, 3719-3728. [2] Zhuang, B., #  Gan, W.P., Wang, #  S.Q., Han, J.P., Xiang, G.X., Li, C.X., Sun, J., Liu, P. (2015). A fully automated sample preparation microsystem for genetic testing of hereditary hearing loss using two-color multiplex allele-specific PCR. Analytical Chemistry, 87(2):1202-1209. [3] Zhuang, B., #  Han, J.P., #  Xiang, G.X., Gan, W.P., Wang, S.Q., Wang, D., Wang, L., Sun, J., Li, C.X., Liu, P. (2016). A fully integrated and automated microsystem for rapid pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. Lab on a Chip 16, 86-95.
Release date NZ
July 28th, 2017
Author
Audiences
  • Postgraduate, Research & Scholarly
  • Professional & Vocational
Edition
1st ed. 2018
Illustrations
44 Illustrations, color; 14 Illustrations, black and white; XXIII, 114 p. 58 illus., 44 illus. in color.
Pages
114
ISBN-13
9789811047527
Product ID
26774194

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