To save live of the chronic blood transfusions patients' as much possible through reduce complications of blood transfusion especially multiple blood transfusions and women's having negative blood group. To determine the prevalence of RBCs allo-antibodies in multiple of blood components transfusions. To identify the common alloantibodies in Jaipur. Improving the safety of blood components transfusions. Management & treatment of women having alloantibodies and their fetus. Blood transfusions management of immunized patients. To avoid hemolytic disease of the newborn cases. To decrease the screening and identification antibodies' cost to allow general patients do it regularly. To improve health sociality and reduce of mortality. To help all women having negative blood grouping to made health family. In the recent scientific progress in the field of accurate diagnosis and continues the competition of companies to produce the latest sensitive devices and special solutions for safe blood transfusion, but transfer of blood components and organ transplants frequently remains causes many immediately and delayed problems. Packed R-B-Cs transfer are 100% a valuable to manage health care resource especially for thalassemics, case of myeloproliferative disorders, hematological disorders, end stage renal failure, sufferer of leukemia and anemia, cancer, and organs transplant case as liver, as well as Kidney and bone marrow. Chronic packed red cells transfer can cause unwanted more complex complications called acute or/and chronic blood- transfusion reactions in a patient that may be cause to death in some cases. Development of allo-antibodies to vary R-B-Cs antigens is very an important immune intermediated delayed hemolytid transfusion reaction (DHTR) that may be causing death in most case especially in children, or old illness. It is a matter of major concern about of multi-transfused cases and in women who had multiple pregnancies.' Allo-immunization to R-B-Cs antigen is one of the major complexities regular of the blood components transfused, particularly in sufferer who are chronically transfusions through his/her life. The elements for allo-immunization are complex and involve three major contributing factors: the R-B-Cs antigen asymmetry between the donor and the recipient, the receiver immune situation, and the Immuno-modulatory impact of the allogenic blood components transfusions on the recipient's immune system. Multiple ethnicity of the population has caused genetic heterogeneity among the population which in turn led to a wide variation of antibody specificity among the population.