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Cytochrome P450

In Vitro Methods and Protocols
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Description

This collection explores detailed experimental protocols necessary for setting up a variety of in vitro cytochrome P450 (CYP) assays that are vital in selecting drug candidates in a drug discovery pipeline. Major factors affecting drug metabolism include CYP expression levels, kinetic parameters for individual CYP enzymes, CYP inhibition and induction, time-dependent inhibition (TDI), CYP stability, non-CYP stability, UDP-glucuronosyltransferases (UGT) stability, excretion mechanisms, and drug-drug interactions (DDI), all addressed in this volume. Written for the Methods in Pharmacology and Toxicology series, chapters include helpful background information on the in vitro assay, a list of all the materials, reagents, and equipment necessary to carry out the assay, a step-by-step protocol, notes containing common and unexpected experimental problems in the assay, as well as references containing important supplementary reading.  Authoritativeand practical, Cytochrome P450: In Vitro Methods and Protocols serves as a key guide for researchers in the area of discovery and development of new medicines. 
Release date NZ
July 10th, 2021
Audience
  • Professional & Vocational
Contributors
  • Edited by Gary W. Caldwell
  • Edited by Zhengyin Yan
Edition
1st ed. 2021
Illustrations
40 Illustrations, color; 54 Illustrations, black and white; XI, 379 p. 94 illus., 40 illus. in color.
Pages
379
ISBN-13
9781071615416
Product ID
34636870

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