Also the hundred years literature is completely silent on the on the analysis of waxes of S. nigrum with respect to its taxonomical detail. Five selected plants of S.nigrum Complex among most authorized taxonomists. Therefore, thorough investigation of epicuticular waxes of these five plants of S. nigrum Complex and its possible utilization in chemotaxonomy for their correct classification. The compounds identified were: Squalene; Tetradecane; Hexadecane; Octadecane; Tritetracontane; Heptadecane; Heneicosane; Ascorbic acid; Eicosane; Phytol; 1,2-Benzenedicarboxylic acid; cis-1-chloro-9-octadecene; Ditetradecyl ether; 2,6-Diisopropylnaphthalene; Cyclodecasiloxane; Spirost-8-en-11-one; Hexadecanoic acid; cis, cis, cis-7,10,13-Hexadecatrienal; 9,9 -Dimethoxybicyclo [3.3.1] nona-2,4-dione; Bis(2-ethylhexyl)phthalate; 2,6,10,14,18,22-Tetracosahexene and 2,6,10,14,18-Pentamethyl-2,6-10,14-18-eicosapentae