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Cell-Free Gene Expression on Hardback
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Cell-Free Gene Expression

Methods and Protocols
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Description

This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Cell‐Free Gene Expression: Methods and Protocols serves as an idealguide for researchers seeking technical methods to current aspects of CFE and related applications.
Release date NZ
January 6th, 2022
Audience
  • Postgraduate, Research & Scholarly
Contributors
  • Edited by Ashty S. Karim
  • Edited by Michael C Jewett
Edition
1st ed. 2022
Illustrations
63 Illustrations, color; 17 Illustrations, black and white; XV, 435 p. 80 illus., 63 illus. in color. With online files/update.
Pages
435
ISBN-13
9781071619971
Product ID
35790119

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