The present study was conducted during the period from January 2010 to December 2010 in south sector of Main out full drain, Nassiriya. Three stations were fixed to achieve the study. The concentration of eight trace metals (cadmium, cobalt, copper, iron, manganese, nickel, lead and zinc) were measured in dissolved and particulate phases of water, exchangeable and residual phases of the sediment and tissues (gills, liver, gut, muscle, ovary and kidney) of two important commercial species of fish Cyprinus carpio L. and Barbus luteus L. In addition, the concentrations of these metals in blood samples of some citizens of study area were measured.The TMs concentration in the particles phase are much more than the dissolved phase.The TMs concentration in the residual phase are much more than the exchangeable phase expect Cd, Pb metals can be attributed to anthropogenic sources.Although fish are particularly good indicators of TMs pollution, it is clear that the different species have different affinities for certain metals, also fish tissues have different affinities for certain trace metals even in the same species.