Biophysical Chemistry explores the concepts of physical chemistry and molecular structure that underlie biochemical processes. Ideally suited for undergraduate students and scientists with backgrounds in physics, chemistry or biology, it is also equally accessible to students and scientists in related fields as the book concisely describes the fundamental aspects of biophysical chemistry, and puts them into a biochemical context. This second edition has been fully updated throughout with novel techniques, with a new chapter on advances in cryo-electron microscopy and exciting new content throughout on big data techniques, structural bioinformatics, systems biology and interaction networks, and artificial intelligence and machine learning. The book is organized in four parts, covering thermodynamics, kinetics, molecular structure and stability, and biophysical methods. Cross-references within and between these parts emphasize common themes and highlight recurrent principles. End of chapter problems illustrate the main points explored and their relevance for biochemistry, enabling students to apply their knowledge and to transfer it to laboratory projects. Key Features: Connects principles of physical chemistry to biochemistry. Emphasizes the role of organic reactions as tools for modification and manipulation of biomolecules. Includes a comprehensive section on the theory of modern biophysical methods and their applications. Dagmar Klostermeier is of the University of Muenster, Germany. Markus G. Rudolph is of the Pharma Research & Early Development Division in the Roche Group, Switzerland.