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ADP-ribosylation and NAD+ Utilizing Enzymes

Methods and Protocols
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Description

This volume focuses on mono-ADP-ribosylation and enzymes that use NAD+ including Sirtuins, PARPs, and bacterial and eukaryotic ADP-ribosyltransferases. The chapters in this book are organized into eight parts, and offer detailed descriptions of key protocols used to study topics such as in vitro techniques for ADP-ribosylation substrate identification; biochemical and biophysical assays of PAR-WWE domain interactions; monitoring expression and enzyme activity of ecto-ARTCs; HPLC-based enzymes assays for Sirtuins; and identifying target RNAs of PARPs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, ADP-ribosylation and NAD+ Utilizing Enzymes: Methods and Protocols is a valuable resource for anyone interested in this developing and expanding field.
Release date NZ
August 11th, 2018
Audience
  • Postgraduate, Research & Scholarly
Contributor
  • Edited by Paul Chang
Edition
1st ed. 2018
Illustrations
51 Illustrations, color; 44 Illustrations, black and white; XIV, 417 p. 95 illus., 51 illus. in color.
Pages
417
ISBN-13
9781493985876
Product ID
27823966

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Books >
Non-Fiction >
Maths & Science >
Biology, life sciences >
Life sciences: general issues >
Genetics >
DNA
Books >
Non-Fiction >
Maths & Science >
Biology, life sciences >
Life sciences: general issues >
Genetics
Books >
Non-Fiction >
Maths & Science >
Biology, life sciences >
Biochemistry
Books >
Non-Fiction >
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Proteins