Excerpt from Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol. 5: April, 1912 I should like to say that the difficulty of demonstrating intestinal entamoebm, either in the living or fixed and stained condition, is much exaggerated. After a little practice it is impossible'to miss them, and if thick slides, which have been-dipped in hot water are used for fresh specimens, the movements of the organism can be readily followed in ordinary room temperatures for two. Or three hours. Except in very cold weather, a warm stage is unnecessary. As to stains, a modifi cation of romanowski is essential, smee, for difierentiation of species, nuclear staining is very important, and I find that one routine method for practically all specimens, V'iz., giemsa's original process, is the best. As you see, with its use one should not mistake either the nature of the organism or its contents. The red blood corpuscles, which in this specimen are lying free in the blood-stained mucus in such quantities, take on an orange-red shade, which they retain after they are englobed, although it gradually becomes paler and dingier as digestion goes oh. One occasionally sees some debris and granular matter extruded by the living organism, but apparently the whole corpuscle, haemoglobin and all, is assimilated by Entamceba histolyti'ca without difficulty and without refuse. About the Publisher Forgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.com This book is a reproduction of an important historical work. Forgotten Books uses state-of-the-art technology to digitally reconstruct the work, preserving the original format whilst repairing imperfections present in the aged copy. In rare cases, an imperfection in the original, such as a blemish or missing page, may be replicated in our edition. We do, however, repair the vast majority of imperfections successfully; any imperfections that remain are intentionally left to preserve the state of such historical works.