The purpose of this case study is to describe how PMTCT services were delivered in our program and to highlight the achievements and challenges of the collaborative efforts to improve PMTCT services in Yunnan over the past eight years. We hope that sharing our program experiences would be valuable for others implementing integrated HIV, syphilis and HBV PMTCT services in resource-limited settings and reach the WHO goal of "virtual elimination" of pediatric HIV and congenital syphilis by 2015.