Revised and updated to provide a more general coverage, this third edition of the text is a guide to the study of structure, activity and mechanism in the field of protein science.

Table of Contents

The three-dimensional structure of proteins; chemical catalysis; the basic equations of enzyme kinetics; measurement and magnitude of enzymatic rate constants; the pH dependence of enzyme catalysis; practical kinetics; detection of intermediaries in reactions by kinetics; stereochemistry of enzymic reactions; active-site-directed and enzyme-activated irreversible inhibitors - affinity labels and suicide inhibitors; conformational change, allosteric regulation, motors and work; forces between molecules, and enzyme-substrate binding energies enzyme-substrate complementarity and the use of binding; energy in catalysis; specificity and editing mechanisms; recombinant DNA technology; case studies of enzyme structure and mechanism; protein engineering; protein stability; kinetics of protein folding; folding pathways and energy landscapes.