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Pre-Field Screening Protocols for Heat-Tolerant Mutants in Rice

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Pre-Field Screening Protocols for Heat-Tolerant Mutants in Rice

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Description

This open access book presents simple, robust pre-field screening protocols that allow plant breeders to screen for enhanced tolerance to heat stress in rice. Two critical heat-sensitive stages in the lifecycle of the rice crop are targeted – the seedling and flowering stages – with screening based on simple phenotypic responses. The protocols are based on the use of a hydroponics system and/or pot experiments in a glasshouse in combination with a controlled growth chamber where the heat stress treatment is applied. The protocols are designed to be effective, simple, reproducible and user-friendly. The protocols will enable plant breeders to effectively reduce the number of plants from a few thousands to less than 100 candidate individual mutants or lines in a greenhouse/growth chamber, which can then be used for further testing and validation in the field conditions. The methods can also be used to classify rice genotypes according to their heat tolerance characteristics. Thus, different types of heat stress tolerance mechanisms can be identified, presenting opportunities for pyramiding different (mutant) sources of heat stress tolerance. 

Author Biography:

Fatma Sarsu Plant Breeding and Genetics Section Joint FAO/IAEA Division Vienna, Austria   Abdelbagi M.A. Ghanim Plant Breeding and Genetics Laboratory Joint FAO/IAEA Division Vienna, Austria   Priyanka Das School of Life Sciences Jawaharlal Nehru University New Delhi, India   Rajeev N Bahuguna School of Life Sciences Jawaharlal Nehru University New Delhi, India   Paul Mbogo Kusolwa Sokoine University of Agriculture Morogoro, United Republic of Tanzania   Muhammed Ashraf Nuclear Institute for Agriculture and Biology (NIAB) Faisalabad, Pakistan   Sneh Lata Singla-Pareek Plant Molecular Biology Group International Centre for Genetic Engineering and Biotechnology New Delhi, India   Ashwani Pareek School of Life Sciences Jawaharlal Nehru University New Delhi, India   Brian P. Forster Plant Breeding and Genetics Laboratory  Joint FAO/IAEA Division International Atomic Energy Agency  Vienna, Austria Ivan Ingelbrecht Plant Breeding and Genetics Laboratory Joint FAO/IAEA Division International Atomic Energy Agency  Vienna, Austria
Release date NZ
August 23rd, 2018
Audience
  • Professional & Vocational
Edition
1st ed. 2018
Illustrations
10 Illustrations, color; 6 Illustrations, black and white; XII, 39 p. 16 illus., 10 illus. in color.
Pages
39
ISBN-13
9783319773377
Product ID
27601901

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