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High Resolution Chromatography

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High Resolution Chromatography

A Practical Approach

Format

Hardback

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High Resolution Chromatography
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Description

The molecular biology revolution has required the development of new chromatographic techniques and the optimization of original techniques to give reasonable quantities of protein at high resolutions. The aim of this volume is to provide the necessary information in most experimental situations to enable rapid and effective purification. The first four chapters deal with the instrumental aspects of high resolution chromatography starting with the initial clean up steps prior to separation in chapter 1. Chapter 2 deals with microscale techniques, then chapter 3 describes the detector technologies that can determine information about the separated molecules. The final chapter in this section cover capillary electrophoresis and its associated techniques. The remaining chapters cover a range of chromatographic procedures based on the interaction of a specific ligand with its target protein or other macromolecule. Some chapters cover non-specific interactions using peptides, inhibitors, and antibodies as the affinity ligand while others focus on specific groups of molecules : oligosaccharides and glycosylated proteins, nucleotide-binding proteins, proteins binding free and chelated metal ions, and DNA binding proteins.
Release date NZ
April 29th, 1999
Contributor
Edited by Paul Millner
Country of Publication
United Kingdom
Illustrations
5 halftones, 70 line figures
Imprint
Oxford University Press
Pages
333
Dimensions
160x241x21
ISBN-13
9780199636495
Product ID
2247516

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