Table of Contents
Benson's Microbiological Applications, Laboratory Manual in General Microbiology, Complete Version, 10th Edition Part 1 Microscopy Exercise 1 Brightfield Microscopy Exercise 2 Darkfield Microscopy Exercise 3 Phase-Contrast Microscopy Exercise 4 Fluorescence Microscopy Exercise 5 Microscopic Measurements Part 2 Survey of Microorganisms Exercise 6 Protozoans, Algae, and Cyanobacteria Exercise 7 Ubiquity of Bacteria Exercise 8 The Fungi: Yeasts and Molds Part 3 Manipulation of Microorganisms Exercise 9 Aseptic Technique Exercise 10 Pure Culture Techniques Part 4 Staining and Observation of Microorganisms Exercise 11 Smear Preparation Exercise 12 Simple Staining Exercise 13 Negative Staining Exercise 14 Capsular Staining Exercise 15 Gram Staining Exercise 16 Spore Staining: Two Methods Exercise 17 Acid-Fast Staining: Ziehl-Neelsen Method Exercise 18 Motility Determination Part 5 Culture Methods Exercise 19 Culture Media Preparation Exercise 20 Preparation of Stock Cultures Exercise 21 Cultivation of Anaerobes Exercise 22 Enumeration of Bacteria: The Standard Plate Count Exercise 23 Slime Mold Culture Exercise 24 Slide Culture: Molds Part 6 Bacterial Viruses Exercise 25 Determination of a Bacteriophage Titer Exercise 26 Burst Size Determination: A One-Step Growth Curve Exercise 27 Isolation of Phage from Flies Exercise 28 Phage Typing Part 7 Enviornmental Influences and Control of Microbial Growth Exercise 29 Temperature: Effects on Growth Exercise 30 Temperature: Lethal Effects Exercise 31 pH and Microbial Growth Exercise 32 Water Activity and Osmotic Pressure Exercise 33 Ultraviolet Light: Lethal Effects Exercise 34 Oligodynamic Action Exercise 35 Evaluation of Alcohol: Its Effectiveness As an Antiseptic Exercise 36 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method Exercise 37 Evaluation of Antiseptics: The Filter Paper Disk Method Exercise 38 Effectiveness of Hand Scrubbing Part 8 Identification of Unknown Bacteria Exercise 39 Morphological Study of Unknown Bacterium Exercise 40 Cultural Characteristics Exercise 41 Physiological Characteristics: Oxidation and Fermentation Tests Exercise 42 Physiological Characteristics: Hydrolytic Reactions Exercise 43 Physiological Characteristics: Biochemical Tests Exercise 44 Use of Bergey's Manual Part 9 Miniaturized Multitest Systems Exercise 45 Enterobacteriaceae Identification: The API 20E System Exercise 46 Enterobacteriaceae Identification: The Enterotube II System Exercise 47 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System Exercise 48 Staphyococcus Identification: The API Staph-Ident System Part 10 Diversity and Environmental Microbiology Exercise 49 Isolation of an Antibiotic Producer: The Actinomyces Exercise 50 Nitrogen Cycle: Ammonification Exercise 51 Symbiotic Nitrogen Fixation: Rhizobium Exercise 52 Free-Living Nitrogen Fixation: Azotobacter Exercise 53 Denitrification: Paracoccus Denitrificans Exercise 54 The Winogradsky Column Exercise 55 Purple Nonsulfer Photosynthetic Bacteria Exercise 56 Sulfate Reducing Bacteria: Desulfovibrio Exercise 57 Bacterial Commensalism Exercise 58 Bacterial Synergism Exercise 59 Microbial Antagonism Part 11, Applied Microbiology Exercise 60 Bacterial Counts of Foods Exercise 61 Bacteriological Examination of Water: Qualitative Tests Exercise 62 The Membrane Filter Method Exercise 63 Reductase Test Exercise 64 Microbial Spoilage of Canned Food Exercise 65 Microbiology of Alcohol Fermentation Part 12 Bacterial Genetics and Biotechnology Exercise 66 Mutant Isolation by Replica Plating Exercise 67 Bacterial Transformation Exercise 68 Polymerase Chain Reaction for Amplifying DNA Exercise 69 Plasmid Isolation Part 13 Medical Microbiology and Immunology Exercise 70 The Staphylococci: Isolation and Identification Exercise 71 The Streptococci: Isolation and Identification Exercise 72 Gram-Negative Intestinal Pathogens Exercise 73 Slide Agglutination Test: Serological Typing Exercise 74 Slide Agglutination (Latex) Test: For S. aureus Identification Exercise 75 Tube Agglutination Test: The Heterophile Antibody Test Exercise 76 White Blood Cell Study: The Differential WBC Count Exercise 77 Blood Grouping Exercise 78 A Synthetic Epidemic Appendix A: Tables Appendix B: Indicators, Stains, Reagents Appendix C: Media Appendix D: Identification Charts Appendix E: The Streptococci: Classification, Habitat, Pathology, and Biochemical Characteristics
Professor and Chair, Consultant Environmental Microbiology
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